mouse glomerular mesangial cells Search Results


mouse glomerular mesangial cell line  (BioResource International Inc)
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BioResource International Inc mouse glomerular mesangial cell line
Effects of resveratrol and klotho gene silencing on concanavalin A (Con A)-induced changes in the protein levels of klotho and SIRT-1, glomerulosclerosis-regulated factors, and reactive oxygen species (ROS) in <t>mesangial</t> cells. ( A ) The mRNA levels of transforming growth factor (TGF)-β, fibronectin, procollagen-III, and collagen I, and ( B ) the relative ROS levels were detected using flow cytometry after preincubation of mesangial cells with 25 μM resveratrol for 2 h, followed by cotreatment with 25 μΜ resveratrol plus 20 μg/mL Con A for 30 min. ( C ) mRNA levels of TGF-β, fibronectin, procollagen-III and collagen I, and ( D ) ROS relative levels were measured using flow cytometry after sequential treatments with klotho siRNA, 25 μΜ resveratrol for 2 h, 25 μΜ resveratrol plus 20 μg/ml Con A for 30 min. Different letters (a, b, and c) indicate significant differences between the groups ( p < 0.05).
Mouse Glomerular Mesangial Cell Line, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse glomerular mesangial cell line - by Bioz Stars, 2026-07
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mouse glomerular mesangial cell complete medium  (Elabscience Biotechnology)
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sv40-rfp-mouse glomerular mesangial cells  (Angio-Proteomie)
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Effects of resveratrol and klotho gene silencing on concanavalin A (Con A)-induced changes in the protein levels of klotho and SIRT-1, glomerulosclerosis-regulated factors, and reactive oxygen species (ROS) in mesangial cells. ( A ) The mRNA levels of transforming growth factor (TGF)-β, fibronectin, procollagen-III, and collagen I, and ( B ) the relative ROS levels were detected using flow cytometry after preincubation of mesangial cells with 25 μM resveratrol for 2 h, followed by cotreatment with 25 μΜ resveratrol plus 20 μg/mL Con A for 30 min. ( C ) mRNA levels of TGF-β, fibronectin, procollagen-III and collagen I, and ( D ) ROS relative levels were measured using flow cytometry after sequential treatments with klotho siRNA, 25 μΜ resveratrol for 2 h, 25 μΜ resveratrol plus 20 μg/ml Con A for 30 min. Different letters (a, b, and c) indicate significant differences between the groups ( p < 0.05).

Journal: International Journal of Molecular Sciences

Article Title: Resveratrol Pretreatment Ameliorates Concanavalin A-Induced Advanced Renal Glomerulosclerosis in Aged Mice through Upregulation of Sirtuin 1-Mediated Klotho Expression

doi: 10.3390/ijms21186766

Figure Lengend Snippet: Effects of resveratrol and klotho gene silencing on concanavalin A (Con A)-induced changes in the protein levels of klotho and SIRT-1, glomerulosclerosis-regulated factors, and reactive oxygen species (ROS) in mesangial cells. ( A ) The mRNA levels of transforming growth factor (TGF)-β, fibronectin, procollagen-III, and collagen I, and ( B ) the relative ROS levels were detected using flow cytometry after preincubation of mesangial cells with 25 μM resveratrol for 2 h, followed by cotreatment with 25 μΜ resveratrol plus 20 μg/mL Con A for 30 min. ( C ) mRNA levels of TGF-β, fibronectin, procollagen-III and collagen I, and ( D ) ROS relative levels were measured using flow cytometry after sequential treatments with klotho siRNA, 25 μΜ resveratrol for 2 h, 25 μΜ resveratrol plus 20 μg/ml Con A for 30 min. Different letters (a, b, and c) indicate significant differences between the groups ( p < 0.05).

Article Snippet: The mouse glomerular mesangial cell line was obtained from the Bioresource Collection and Research Center, Food Industry Research and Development Institute (cat. No. 60366; Hsinchu, Taiwan).

Techniques: Flow Cytometry